The Fact About Pos88 That No One Is Suggesting
The Fact About Pos88 That No One Is Suggesting
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, 2006; Sawaya and Kraut, 1997; Figure one—figure health supplement 1), we envisioned the mutational landscape of DHFR to generally be constrained through the necessity to adopt these various conformations. Additionally, prior perform experienced advised DHFR is impacted by cellular constraints for instance protein quality Management (Bershtein et al., 2013) plus the build-up of a toxic metabolic intermediate (Schober et al., 2019). We as a result envisioned deep mutational scanning to reveal a hugely constrained mutational landscape for DHFR that may distinction With all the mutational tolerance observed in other systems.
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Protein mutational landscapes are shaped through the mobile setting, but vital things and their quantitative effects tend to be not known. Here we exhibit that Lon, a high quality Management protease naturally absent in widespread E. coli expression strains, greatly reshapes the mutational landscape in the metabolic enzyme dihydrofolate reductase (DHFR). Selection under situations that resolve remarkably Lively mutants reveals that 23.
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(2020) Altered expression of an outstanding Manage protease in E. coli reshapes the in vivo mutational landscape of a design enzyme
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